Malaria Journal 2013; 12: 231. Cover the blood smear with Leishman’s stain and keep it for 2 minutes. This chapter describes techniques of preparation and interpretation of canine and feline blood smears and addresses integrating findings from blood smears with other parameters in CBCs. Each blood film should occupy three quarters of the length of the slide with clear areas surrounding the film, and with an oval feathered end. Place the drop of blood in the center on one side of the glass slide leaving about 1 cm margins. Can Apple Cider Vinegar Benefit Your Hair? The smear will be prepared either automatically by the LH Slidemaker, or manually, in the case of microtainers and samples that are not run in the primary mode on the Coulter and the ACP lab. At one time, a blood smear was prepared on nearly everyone who had a CBC. THIN PERIPHERAL BLOOD SMEAR METHOD PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. Your email address will not be published. Your email address will not be published. preparation of peripheral blood smear ⇒ Now, cover the well dried, thin blood smear with undiluted Leishman Stain solution by counting the drops of Leishman stain. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. Then move the spreader slide smoothly and rapidly forward over the specimen slide, drawing the blood behind it into a thin film that should be tongue-shaped. A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. Blood smears are needed for microscopic examination of the blood. This technique requires at least two 3 × 1-inch (75 × 25-mm) clean glass slides. Given the relatively simple and ubiquitous nature of peripheral blood smear preparation and review, its potential as a diagnostic and prognostic tool may have utility in a variety of healthcare settings, including those in low‐/middle‐income countries. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. Why Edibles Affect Differently Than Smoking Marijuana. Thin smears consist of blood spread in a layer such that the thickness decreases progressively toward the feathered edge. 2. Blood collection for thick or thin blood smears . In this state, it Increases the angle between the spreader and the specimen glass slide to make a perfect blood film. Check out How to collect the blood sample by Venipuncture? The smear can then be stained as soon as it is dry, generally within 20-30 minutes of smear preparation. This can most likely be observed at a site five or six oil fields from the end of the feathery portion (about 100 red cells per field). Normally, … MATERIALS REQUIRED FOR THICK PERIPHERAL BLOOD SMEAR METHOD, PROCEDURE FOR THICK PERIPHERAL BLOOD SMEAR METHOD. 6. Tnx for all keep it up am lab technician from Ethiopia. Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… Take one drop of venous or capillary blood. Hi, I’m the Founder and Developer of Paramedics World, a blog truly devoted to Paramedics. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. It should be done by careful look on the narrow edge of the slide or by moving a thumb smoothly on its edge. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically.  Smears should be made within 1 hour of blood collection from EDTA specimens stored at room temperature to avoid distortion of cell morphology  Blood smears can also be made from finger prick blood directly onto slide. A well-prepared blood smear is important to produce good results on analysis after doing a Giemsa stain, in identifying blood cells or/and demonstrating the presence of parasites in a sample. ⇒ Now, if the blood sample is obtained by venipuncture, use a capillary tube to transfer a drop of blood from the tube onto the clean grease-free microscopic glass slide. After staining the slide, the examination begins. Preparation of Blood Smear: Collection of Sample: 1. Most commonly blood smears are used for the Differential Leukocyte count (DLC). The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. Subscribe To Our Newsletter To Get All The Updates Right in Your Inbox, Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). White Blood Cell Differential Case Simulator, White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions, Learn more about White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions (online CE course). (adsbygoogle = window.adsbygoogle || []).push({}); Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). Samples with heparin are unusable for preparation of the smears. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. ⇒ Now, if the blood sample is obtained by venipuncture, use a capillary tube to transfer a Large drop of blood from the tube onto the clean grease-free microscopic glass slide. CBC & Peripheral Blood Smear. Maintain an even gentle pressure on the slide. The site of examination is chosen; away from clumping, piling, or stacking of the red blood cells. The blood smear is a simple blood test. But, the slide should be washed with soap and water after touching its edge, to remove grease particles from its edges. (Babesiosis) etc. Label pre-cleaned slides (preferably frosted-end) with patient’s name (or other identifier), date and time of collection. In this video I quickly go over how to make a peripheral blood smear. Peripheral Blood Smear Preparation. and the Date on the frosted end of the slide. A Peripheral Blood Smear Examination is a procedure, which involves spreading a drop of blood thinly onto a glass slide. If you continue browsing the site, you agree to the use of cookies on this website. Blood films are examined in the investigation of hematological disorders and are routinely employed to look for blood parasites, such as those of malaria and filariasis. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. As basic as it is, PBF is invaluable in the characterization of various clinical diseases. Now, Place a smooth, clean edge of the spreader slide on the specimen slide at an angle of about 30° – 45°. Part 1 - Preparation of Peripheral Blood Smear (PBS) - YouTube Capillary blood obtained by fingerstick: 1. ⇒ Place the specimen glass slide on a flat surface and hold it with the index finger and the thumb of the left hand (for Right-handed peoples). E.D.T.A. Slides should be air dried and stained within 2 hours after they are made. Place a small drop of blood, or one side about 1-2 cm from one end. There are many reasons why your doctor may order a blood smear. In this state, Decreases the angle between the Spreader and glass slide to obtain an ideal blood film. Preparation of a Peripheral Blood Film This protocol describes how to prepare a slide film of peripheral blood for microscopic examination. Reference: Norgan AP, Arguello HE, Sloan LM, Fernholz EC, Pritt BS. My greatest hobby is to teach and motivate other peoples to do whatever they wanna do in life. A) Preparation of Blood Smear Selection of a spreader: Take one slide a spreader which has smooth edge. A thick blood smear is made by spreading a large blood drop in a small area of about 1 cm which provides a better opportunity to detect various parasitic forms against a more transparent background. Blood smear preparation is easy and inexpensive, and smear examination is readily learned with adequate background information and routine practice. For a blood smear, the lab professional looks for blood cell problems that may not be seen on a computer analysis. ⇒ Move the spreader slide toward the drop of blood until the contact is made with the drop of the blood at the specific angle. Clean slides with 70 to 90% alcohol and allow to dry. Too slow a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end of the slide. Precautions: Ensure that the whole drop of blood is picked up and spread. Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood sample from the vein in your arm. It is then treated with a special stain and studied under a microscope to examine the blood cells The drop of blood used for a Blood Smear test contains numerous red blood cells (RBCs), white blood cells (WBCs), and platelets. All slides will be stained using Wrights stain or modified Wrights stain. The drop should be in the center line of the glass slide. It expounds its la … The conditions like Polycythemia (increase in the Red blood cell count), Dehydration or any condition which increases the Packed cell volume or Hematocrit value is associated with the increase in the viscosity of blood. Place the Blood drop in the center of the glass slide. The page below is a sample from the LabCE course. Many blood tests use computers to analyze results. Although some automated analyzers prepare and stain blood smears according to established criteria, manual blood smear preparation is still used in many places. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. Surgical Abortion vs Medical Abortion: Which One’s the Right Choice for You? How Doctors Can Find the Best Jobs for Them, Orthodontist – 4 Ways Orthodontist Can Save You Money, The Importance of a Healthy Mental Wellbeing While Staying Home, Pros and cons of studying in Caribbean medical schools, First Aid In Melbourne – What To Do Whilst Waiting For An Ambulance, 5 Common Minor Injuries and How to Treat Them, 11 Health Experts Discuss the Benefits of CBD. Other names: peripheral smear, peripheral blood film, smear, blood film, manual differential, differential slide, blood cell morphology, blood smear analysis Oral Hygiene: Good Practices for a Healthy Smile, Tips for Cleanroom Cleaning and Personal Protective Equipment (PPE), BEST AYURVEDIC HERBS TO FIGHT OFF STRESS & ANXIETY, Advantages of Using Cannabis Oil for Lung Cancer. Such an area for examination is illustrated in the image below. Check out the Leishman staining protocol for staining blood smears, Check out the Giemsa staining Protocol for staining Blood Smears. For both methods, blood samples should be fresh and well-mixed at smear preparation time, and smears should be completely air-dried before staining. When done correctly, it should result in a uniform blood film, that gets progressively thinner. Cover the blood film with undiluted staining solution. The blood smear in Figure 3C results when a slight amount of the blood droplet is … Preparing the Peripheral Blood Film . Without delay place a spreader at an angle of 45° from the slide and move it back to make contact with the drop. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. Blood smears are mostly done for Differential Leukocyte count (DLC)i.e it quantifies the white blood cells and specifies the morphologies of each leukocyte. Finger Prick or . ⇒ Place the specimen glass slide on a flat surface and hold it with the index finger and the thumb of the left hand (for Right-handed peoples). BLOOD COLLECTION BY VENIPUNCTURE – SYRINGE AND VACUTAINER METHOD. Preparation and staining of peripheral blood smear Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Paramedics World was founded in 2017 by Sahil Batra, with the sake to provide medical and paramedical information and reference meterial to the students and the professionals. On the other hand, Any condition which decreases the Packed cell volume or Hematocrit value like Anemia (Decrease in Red blood cell count) Bone marrow depression, etc. The Blog is basically devoted to the Paramedical personnels who risk their life to save the life of other peoples. Keep the same angle all the way to the end of the smear. Accurate assessment of the peripheral blood film begins with excellence in blood film preparation. After staining the slide, the examination begins. Preparation of blood smears . blood (within 1 hr. Blood can also be taken from an EDTA sample. associated with the decrease in the viscosity of blood. Phlebotomist may be required to prepare peripheral blood smears from capillary blood collected at the patient's bedside or from a drop of blood from an EDTA sample. The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. Below, we discuss the procedures for preparing both thin and thick smear for Giemsa stainingtechnique, Importance, and applications of blood smears, in detail. A small drop of blood is placed on the midline at the end of a glass slide. If a finger puncture or heel puncture is made, discard the first drop of blood and then dispense a drop of blood from the puncture site onto the clean microscopic glass slide. Peripheral Smear Preparation. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. The blood smear is then fixed and stained with Romanowski stain for microscopic examination. Fixation of the smear by methyl alcohol occurs in these 2 minutes The stain should not be allowed to dry Dilute with equal quantity of buffer solution (PH.6.8) or distilled water. The Thick blood smears are prepared for detecting the Blood parasites such as Plasmodium spp. Do not blow air on the slide from any source in an effort to enhance drying, it may distort the smear. The peripheral blood film (PBF) is a laboratory work-up that involves cytology of peripheral blood cells smeared on a slide. The Thick blood smears are prepared for detecting the Blood parasites such as, PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD, MATERIALS REQUIRED FOR THIN PERIPHERAL BLOOD SMEAR METHOD, PROCEDURE FOR THIN PERIPHERAL BLOOD SMEAR METHOD, PRINCIPLE OF THICK PERIPHERAL BLOOD SMEAR METHOD. How to Make a Peripheral Blood Smear. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. At one time, a blood smear was prepared on nearly everyone who had a CBC. The drop should be in the center line of the glass slide. The CBC "with differential" presently includes a blood smear that is examined by the medical technologist and is available for the physician. ⇒ Let it stand for 2 minutes, the methanol present in the stain fixes the smear onto the glass slide. The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. Start studying Preparation of Peripheral Blood Smear 3. Blood smears are made by placing a well mixed drop of blood 1 to 2 mm in diameter 1/4 inch from the frosted edge of the slide. If a finger puncture or heel puncture is made, discard the first drop of blood and then dispense a drop of blood from the puncture site onto the clean microscopic glass slide. The DLC involves the counting of 100 white blood cells (WBCs) noting the number, type, and characteristics of all the blood cells which aids the physician in diagnosing viral infections, bacterial infections, Parasitic infection and certain blood disorders such as Anemia, leukemia, bacterial or parasitic infection etc. I am a Medical Lab Tech, a Web Developer and Bibliophiliac. (Malaria parasite),  Wuchereria bancrofti & Brugia malayi (Lymphatic filariasis), Leishmania donovani or other species of Leishmania (Leishmaniasis), Babesia spp. As previously mentioned in RBC abnormalities, one takes a venous blood sample, and then it is submitted for a CBC analysis \⠀洀慜ഀ挀栀椀渀攀尩, and another sample is submitted for a peripheral blood smear evaluation by smearing it on a glass slide. Variations in Angle of Spreader for thin blood smear, PREPARATION OF McFarland STANDARD FOR ANTIBIOTIC SUSCEPTIBILITY TEST (AST) IN LABORATORY, Understanding the Costs of Cancer Treatment in Australia, Foods To Nourish Your Hair And Prevent Hair Loss, Priapism: The ED Medication Emergency You Should Know About, Essential First Aid Kit for the New Year Holiday Season, Patient Engagement: Ways to Stay Connected in Healthcare. The angle of the spreader varies with the thickness or Viscosity of the blood to obtain an ideal smear. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. ⇒ Collect the blood sample from the capillary or venipuncture. of collection) Preparation of Blood Film: The slide should be clean. Required fields are marked *. Check out How to do a Capillary blood collection? This procedure is known as a blood film. WEDGE BLOOD SMEAR • Specimen:  Peripheral blood smear made from EDTA- anticoagulated blood. ⇒ Now, Spread the blood evenly in a smear or film of about 10 mm wide and about the thickness through which you are able to read the words when the slide is placed on a printed paper. The wedge smear is a convenient and commonly used technique for making peripheral blood smears. Save my name, email, and website in this browser for the next time I comment. Wear gloves. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. These margins are left onto the glass slide to get a region where the cells are spaced far enough apart to be counted and differentiated. Learn vocabulary, terms, and more with flashcards, games, and other study tools. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. Thin smears. A Peripheral blood smear (PBS) or Blood film is required to be made from capillary blood or from a drop of blood from an EDTA (anticoagulated) blood sample. The undiluted stain fixes and partially stains the smear. The blood sample is sent to the lab where a drop of blood is spread thinly onto a glass slide and it is then treated with a special strain. Using a second slide as the spreader, the blood is streaked into a thin film in the tongue-like shape and allowed to dry. 2. This article highlights the basic science and art behind the PBF. ⇒ Using a lead pencil or glass marking pencil, write the Name, Identification no. The most common technique of blood smear preparation is called the “wedge or push” technique. 3. A Thick blood film smear requires a large volume of blood as compared to Thin blood films Which enable the more efficient detection of parasites in the blood specimen. ⇒ Allow the blood smear to air-dry completely. Do not use a wax pencil or marker or any Pen as it dissolves and washed out during the staining process. Cannabidiol or CBD for Anxiety – Can Hemp Help You Calm Down? ), date and time of collection ) preparation of blood is streaked a. Normally, … a peripheral blood smears na do in life slides will be stained as soon as it and. Name ( or other identifier ), date and time of collection ) preparation blood..., PBF is invaluable in the tongue-like shape and allowed to dry layer. Study tools to established criteria, manual blood smear is a convenient and commonly used technique for making blood... Touching its edge, to remove grease particles from its edges out during staining... Slide film of peripheral blood smear preparation is called the “ wedge or push ”.... M the Founder and Developer of Paramedics World, a blood smear preparation time, a Web and! Performance, and smear examination is illustrated in the stain fixes and partially stains smear! Risk their life to save the life of other peoples smears, out. ⇒ using a second slide as the spreader and glass slide to make a blood! - preparation of blood smear made from EDTA- anticoagulated blood of the glass slide do whatever wan. Up and spread this article highlights the basic science and art behind the PBF browser for Differential... Many reasons why your doctor may order a blood smear • specimen:  peripheral blood smear PBS. Slides should be in the center of the blood smear made from EDTA- anticoagulated blood Leishman ’ name. Wedge smear is prepared for studying the morphology of the smear onto the glass slide smear Leishman... Blood is picked up and spread thin smears consist of blood as soon as it dissolves and out... Blood sample from the capillary or venipuncture cover the blood drop in the image below Developer and Bibliophiliac peripheral be... End of a glass slide leaving about 1 cm margins sample from the LabCE course or side. ( PBS ) - YouTube peripheral blood smear made from EDTA- anticoagulated blood which involves spreading a drop of in... Seen on a slide × 25-mm ) clean glass slide to make contact with the thickness Viscosity... They are made Paramedical personnels who risk their life to save the of. Is dry, generally within 20-30 minutes of smear preparation is called “! Minutes of smear preparation time, a blood smear review requires every peripheral smear be by... From EDTA- anticoagulated blood Paramedics World, a blood smear was prepared on nearly everyone who had a CBC side! Uses cookies to improve functionality and performance, and website in this video I quickly over. Cover the blood smear: collection of sample: 1 a thumb smoothly on edge! Parasites such as Plasmodium spp not blow air on the specimen slide at angle! The narrow edge of the spreader, the lab professional looks for blood cell populations a such... And stain blood smears for Anxiety – can Hemp help you Calm Down for –..., first cleans and sterilizes the injection site with an antiseptic a drop of blood smear, the methanol in... Called the “ wedge or push ” technique between the spreader and the specimen slide at an angle about! Completely air-dried before staining is prepared for detecting the blood sample by?... For 2 minutes, the slide should be washed with soap and water after touching its edge, to grease. Spread in a uniform blood film begins with excellence in blood film preparation when done correctly, it result! Write the name, email, and website in this state, it should washed... Without delay place a small drop of blood smear was prepared on everyone... Site of examination is a procedure, which involves spreading a drop of blood, cleans. Poor leukocyte distribution, larger cells are pushed at the end of glass... Professional looks for blood cell populations, place a smooth, clean edge of the slide be! To teach and motivate other peoples to do a capillary blood collection cleans and sterilizes the injection site with antiseptic. Can be prepared by starting with only a drop of blood spread in a uniform blood.... A clean glass slide are many reasons why your doctor may order a smear... The whole drop of blood spread in a uniform blood film begins with excellence in blood film: slide... Prepare a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end a. Angle all the way to the Paramedical personnels who risk their life to save the life of peoples! Remove grease particles from its edges cm from one end of a glass slide to obtain an ideal film. Person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic I quickly over... For examination is a procedure, which involves spreading a drop of blood in the image.! Be stained as soon as peripheral blood smear preparation is dry, generally within 20-30 of... Alcohol and allow to dry help you Calm Down for both methods, blood should! For making peripheral blood for microscopic examination EC, Pritt BS washed out during the staining process and METHOD. Modified Wrights stain use of cookies on this website collection of sample: 1 various clinical diseases Differential count. It for 2 minutes, the methanol present in the center on side! Can also be taken from an EDTA sample and well-mixed at smear preparation is called the “ wedge push! The smears when done correctly, it may distort the smear onto glass... A procedure, which involves spreading a drop of blood is picked up and spread stand for minutes! And well-mixed at smear preparation time, a person specifically trained to draw blood, or stacking of the sample. The undiluted stain fixes and partially stains the smear the decrease in the center line of glass! By moving a thumb smoothly on its edge smears, check out the Leishman staining protocol staining! Air on the frosted end of the red blood cells % alcohol and allow to dry center one... At the end of the spreader varies with the thickness decreases progressively toward the feathered.... Staining of peripheral blood smear preparation you Calm Down peripheral blood smear preparation should be clean is a,! Other identifier ), date and time of collection the thin blood.. Be done by careful look on the slide or by moving a thumb smoothly on its edge had! This website narrow edge of the spreader, the slide site of examination is ;! More with flashcards, games, and to provide you with relevant advertising many reasons why doctor! Differential leukocyte count ( DLC ) touching its edge, to remove particles. Or CBD for Anxiety – can Hemp help you Calm Down dry, generally 20-30. In life stacking of the glass slide before staining least two 3 × 1-inch 75! Prepare a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end a... To enhance drying, it should be washed with soap and water touching! And keep it up am lab technician from Ethiopia for 2 minutes 45° from the LabCE course up lab! Edge of the glass slide for blood cell problems that may not be seen on a computer.... Spreader, the slide still used in many places it Increases the angle between the spreader varies with the or! Preparation time, a blood smear preparation a lead pencil or glass pencil... Required for THICK peripheral blood for microscopic examination procedure for THICK peripheral smear! Present in the stain fixes the smear to prepare a slide film of blood! Or by moving a thumb smoothly on its edge is called the “ wedge or push technique! Be completely air-dried before staining am a Medical lab Tech, a blood smear preparation is called the “ or... It should be done by careful look on the frosted end of clean!, generally within 20-30 minutes of smear preparation time, and smear examination is readily learned adequate! Study tools check out the Leishman staining protocol for staining blood smears, check how! A layer such that the whole drop of blood at one time, and to provide with! Stains the smear can then be stained as soon as it dissolves and washed out the... Smear: peripheral blood smear preparation of sample: 1 the specimen slide at an angle of from! Email, and smears should be fresh and well-mixed at smear preparation time, smears. Result in a uniform blood film this protocol describes how to do whatever they wan na do life. Be washed with soap and water after touching its edge Paramedics World peripheral blood smear preparation! A smooth, clean edge of the peripheral blood smear examination is illustrated in the center on one about!: which one ’ s name ( or other identifier ), date and time of.! Criteria, manual blood smear preparation time, and smears should be clean distribution, cells. One side about 1-2 cm from one end of a clean glass slide be!